Technical
information on Affinity Purified Antibodies, F(ab')2 and
Fab' fragments
Whole antibodies
Affinity chromatography is used to isolate antibodies
to defined antigens. Antigen coupled to a solid-phase matrix serves
as immunoabsorbent, and absorbed antibodies are eluted free of contaminating
plasma proteins.
Since affinity purified antibodies are free of unwanted
antibodies and other proteins, they are ideal markers of antigen recognition,
when conjugated with fluorochromes, biotin, or enzymes. Affinity purified
antibodies are supplied as liquids and contain 0.01M phosphate, 0.15M
NaCl, pH 7.2 with 0.1% sodium azide.
F(ab')2 fragments
To eliminate the possibility of non-specific tissue
reactions caused by Fc-receptors, the affinity purified antibodies
are selectively digested with enzymes, that remove the Fc part of
the molecule.The resulting F(ab')2 antibody fragments can
then be labelled with fluorochromes, biotin, or enzymes to produce
conjugates that cause no non-specific reactions on tissues and plasma
membranes. F(ab')2 fragments are supplied as liquids and
contain 0.01M phosphate, 0.15M NaCl, pH 7.2 with 0.1% sodium azide.
Fab' fragments
For studies that require non-crosslinking antibodies,
PROTOS IMMUNORESEARCH offers monovalent Fab' antibody fragments. These
low molecular weight (i.e.3.5S) fragments show increased tissue or
cellular penetration. They are available with antibody activities
to human immunoglobulins and light chains and to a variety of animal
immunoglobulinsFab' fragments are supplied as liquids and contain
0.01M phosphate, 0.15M NaCl, pH 7.2 with 0.1% sodium azide.
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Protos Immunoresearch
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875A Cowan Road, Burlingame, CA 94010
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Tel: 650 652-3450
Fax: 650 652-3451
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F(ab')2 and Fab' fragments
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